The ratio of cell mass to volume defines the cell mass density (CMD), which is also a measure of macromolecular crowding and concentrations of all proteins. Using the fluorescence eXclusion method (FXm) and quantitative phase microscopy (QPM), ...
Specifically, we present quantitative histograms of individual cell mass characterizing the hypertrophic effect of high glucose in a mesangial cell model. In addition, we show that in an epithelial cell model observed for long periods of time, the mean squared displacement data reveal specific information about cellular and subcellular dynamics at various characteristic length and time scales.
Mass tensor molecular dynamics (MTMD) aims to increase the time step by augmenting the Hamiltonian with a position-dependent mass matrix. Higher masses are assigned to modes with fast vibrations.
As we can see from the illustration, Cell Mass Dynamics has many fascinating aspects to explore.
- Mass quantification is demonstrated with picogram resolution on both adherent and non adherent cells as well as single cells and colony-forming cells. The flexibility and speed of the LCI is applied to assess the sensitivity of four human breast cancer lines (BT-474, MDA-MB-231, SK-BR-3, MCF-7) to the HER2-directed monoclonal antibody trastuzumab (Herceptin) and to the growth dynamics of human pluripotent stem cells (HSF1) differentiating under retinoic acid treatment.
- It has been recently observed that single mammalian cells fluctuate their mass in a timescale of seconds5. This result challenges central and longstanding cell growth models, according to which cells increase their mass either linearly or ...
- The measurement of zero-line length has emerged as a novel biomarker for characterizing cell mass transfer. The sensitivity of phase change measurements is demonstrated. The noise quality of input images obtained with incoherent (hiQPI) and coherent (QPI) methods is compared.
- Fast fluctuations of a cell's mass. The inset (left plot) illustrates the dynamics of a cell's total mass over an extended period of time (~hours). The left plot corresponds to a zoom in of the previous inset in which fluctuations of a cell's mass are visible.
